Part:BBa_K4806218:Design

CYPCamC gene for expression in the mitochondria for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 228
  Illegal PstI site found at 409
  Illegal PstI site found at 918
  Illegal PstI site found at 1190
  Illegal PstI site found at 1914
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 228
  Illegal PstI site found at 409
  Illegal PstI site found at 918
  Illegal PstI site found at 1190
  Illegal PstI site found at 1914
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 424
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 228
  Illegal PstI site found at 409
  Illegal PstI site found at 918
  Illegal PstI site found at 1190
  Illegal PstI site found at 1914
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 228
  Illegal PstI site found at 409
  Illegal PstI site found at 918
  Illegal PstI site found at 1190
  Illegal PstI site found at 1914
  Illegal NgoMIV site found at 2935
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. PAR-promotor (A1-B1) (BBa_K3002010)^*
• 1. mtTP70C-transit peptide (B2) (BBa_K4806011)
• 2. CYPCamC gene (B3-B4) (BBa_K4806002)
• 3. HA-tag (B5) (BBa_K3002017)^*
• 4. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B1-B2 (BBa_K3002302)^*
• 2. Connector B2-B3 (BBa_K3002303)^*
• 3. Connector B4-B5 (BBa_K3002304)^*
• 4. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.